Abstract: The objective of study was to establish the quality standard for Hongzhiguke Powder.Rhei Radix et Rhizoma,Phellodendri Chinensis Cortex,Uncariae Ramulus Cumuncis were identified by ThinLayer chromatography (TLC).And the high performance liquid chromatography (HPLC) was applied for the determination of Emodin and Chrysophanol in the Hongzhiguke Powder.The experiment results showed that TLC spots were clear and the blank without interference. Emodin was linear with peak area score when its sample size was within the range of 1.2138.6 μg/mL (r =0.9999),the average recovery was 99.6% (RSD =0.72%,n=6);Chrysophanol was within the range of 2.5380.8 μg/mL (r =0.9991),the average recovery was 99.1% (RSD=0.64%,n=6).Finally,it was concluded that the established methods are simple,reliable and accurate,and can be applied as the quality control method of Hongzhiguke Powder.

Key words: Hongzhiguke powder, emodin, chrysophanol, TLC, HPLC, quality standards