Abstract: To optimize flavonoids extraction condition .With the sum of licorice,quercetin,kaempferol,apigenin as an indicator,response surface methodology was adopted to optimize flavonoids extraction condition by taking enzymatic hydrolysis temperature,pH and dosage.nzymatic hydrolysis temperature 51.21 ℃,pH 4.88,dosage 0.53 mg/mL,under this condition,the predicted value of flavonoids extraction was 25.78 μg/5g,and the test value was 25.55 μg/5g,.the optimal extraction condition were Stable and reliable.

Key words: Rosa laevigata Michx, flavonoids, response surface methodology, HPLC