Abstract: To investigate the effect of methanol extract of Phyllanthus urinaria (P.U.) on αamylase and αglucosidase inhibitory activity in vitro.The inhibitory effects of methanol extract of P.U.on the activity of αamylase and αglucosidase were examined by using pnitrophenylβDgalactopyranoside (PNPG) and starch as substrate respectively.In the experimental dose range,methanol extract of P.U.of αamylase and αglucosidase inhibitory effects were with the increase of the concentration and increased the inhibition and IC50 values were 0.91mg/mL and 0.46 mg/mL.P.U.methanol extract can inhibit the activity ofαamylase and αglucosidase in vitro.

Key words: Phyllanthus urinaria, methanol extract, &alpha, amylase, &alpha, glucosidase