Abstract: The aim of the present study was to identify the different endogenous metabolites in hemolytic blood deficiency rats,and subsequently compare the different enriching-blood mechanisms of Angelica sinensis body and Angelica sinensis tail.Angelica sinensis body and Angelica sinensis tail were used to intervene the hemolytic blood deficiency rats induced by N-acetyl phenyl hydrazine (APH).Metabolomic fingerprints of rat serum samples were determined by ultrahigh-pressure liquid chromatography-quadrupole-time of flight mass spectrometry (UPLC-Q-TOF/MS).The data sets were then imported and subjected to multivariate statistical analysis and univariate statistical analysis using SIMCA-P14.0.Compared with normal group,the levels of succinate,uracil,orotice acid,cytidine,uridine,cholic acid,pyroglutamate acid,cytosine,deoxyuridine,3-methylglycine,and methyladenosine were significantly (P< 0.05) elevated in model group,while the levels of L-glutamine,l-tryptophan,isoleucine,PC(16∶0/16∶0),and L-prolinein were significantly (P< 0.05) decreased,indicating the hemolytic blood deficiency model was built successfully.In addition,the abnormal contents of serum metabolites could be turned over after the treatment of Angelica sinensis body and Angelica sinensis tail,and the Angelica sinensis body had better effects.On the basis of these metabolites,it appeared that the pyrimidine,amino acid metabolism,fatty acid metabolism and energy metabolism could be regulated by Angelica sinensis body and Angelica sinensis tail.

Key words: Angelica sinensis body, Angelic sinensis tail, enriching blood mechanism, metabolomic