Abstract: The constituents were isolated and purified from 95% ethanolic extract of Dalbergia odorifera by silica gel chromatography and HPLC repeatedly,and the structures were identified by spectra analysis and comparison of literature data.Nineteen compounds were isolated from the heartwood of Dalbergia odorifera including four sesquiterpenoids and fifteen flavonoids.The structures were identified as 3′-hydoxymelanettin (1),sativanone (2),pinocembrin (3),6aR,11aR-medicarpin (4),3′-hydroxy-2,4,5,-trimethoxydalbergiquinol (5),rel-(3S,6R,7S)-3,7,11-trimethyl-3,6-epoxy-1,10-dodecadien-7-ol (6),rel-(3R,6R,7S)-3,7,11-trimethyl-3,7-epoxy-1,10-dodecadien-6-ol (7),7,3′,5′-trihydroxyflavanone (8),5,7-dihydroxy-2′,4′-dimethoxyisoflavanone (9),R(+)-4-methoxydalbergione (10),nerolidol (11),6-methyl-2-(5-methyl-5-vinyltetrahydrofuran-2-yl)hept-5-en-2-ol (12),bolusanthin IV (13),4-methoxymedicarpin (14),5,7-dihydroxy-2′,3′,4′-trimethoxyisoflavanone (15),5,7-dihydroxy-4′-methoxyisoflavanone (16),acacetin (17),7-methoxy-3′,4′-dimethoxyisoflavanone (18) and (6aR,11aR)-variabiin (19),respectively.Compounds 8-13 and 15-18 are obtained from Dalbergia odorifera for the first time.Inhibitory effects of the isolated compounds 1-19 on the LPS-induced NO production in RAW 264.7 cells were also evaluated and compounds 1-3,10,15 and 18 showed good inhibitory activities.The IC₅₀ value of compounds 2 and 3 were (34.9±5.4),(22.5±2.7) μM,respectively.

Key words: Dalbergia odorifera T.Chen, Heartwood, Sesquiterpenoid, Flavonoid, Anti-inflammatory