Abstract: To investigate the effect of sapindus-saponin on the proliferation of HepG2 cell lines.HepG2 cell lines were treated by different doses of sapindus-saponin.HepG2 cell viability were evaluated by MTT assay,cell change in morphology was observed by microscope;Fluorescence flow cytometry(FCM)was applied to detect cell cycle and cell apoptosis；qPCR was used to detect the levels of NF-κB and Caspase3.The growth of human hepatocellular carcinoma HepG2 Cells were inhibited after Sapindus-saponin (25-100 μg/mL) treated for 12 h,24 h and 36 h．Meanwhile,morphological changes of cells were obvious.FCM assay indicated that apoptosis peak appeared evidently and HepG2 Cells were atrested in G₀/G₁ phase;the Caspase3 expression were up regulated,and the NF-κB expression were down regulate with the sapindus-saponin concentration increased.In summary,Sapindus-saponin could inhibit the proliferation of HepG2 Cells and induce their apoptosis.

Key words: sapindus-saponin, HepG2 cells, proliferation and apoptosis