Abstract: To study the chemical constituents and the anti-cancer activities from the EtOH extract of Apium graveolens L.Several chromatographic methods were used to separate the chemical constituents，of which structures were identified by IR,MS and NMR.Anti-cancer activities experiments were performed with the monomer compound using the MTT method.Three compounds were isolated from the the EtOH extract of Apium graveolens L and their structures were identified as dicarbamate AG-01(1),bergapten (2) and apigenin (3)．Compound 1 was a new compound.All the compounds were tested for their cytotoxicities against Bel-7402 and SGC7901 cells using the MTT method，and compound 1 exhibited significant cytotoxic effect to Bel-7402 and SGC7901.The IC₅₀ of compound 1 to SGC7901 is 29.6 μM and to Bel-7402 is 32.4 μM.

Key words: Apium graveolens L., chemical constituents, dicarbamate AG-01, cytotoxicity