Abstract: This paper reported the chemical constituents and anti-bacterial activties of Leontopodium haplophylloides Hand.-Mazz.Seventeen compounds were isolated from Leontopodium haplophylloides by column chromatography on silica gel,ODS,Sephadex LH20 and identified by chemical and special analysis.Their structures were elucidated as caffeic acid (1),phenethyl caffeate (2),protocatechuic acid (3),tyrosol (4),p-hydroxybenzoic acid(5),dibutyl phthalate(6),isoscopoletin(7),7-(2,3-epoxy-3-methyl-3-butyloxy)-6-methoxycoumarin(8),apigenin (9),vitexin (10),5-hydroxy-4′,7-dimethoxy-6,8-dimethyl flavones (11),5,7-dihydroxy-8-methoxyflavone (12),kaempferol 3-glucorhamnoside (13),methyl flavogallonate (14),3,5,7,3′,4′-pentahydroxyflavone (15),luteolin (16),galangin (17),among which compounds 1-16 were obtained from this plant for the first time.After antibacterial test,1,2,4-6,9,10 and 13-17 have strong inhibitory effects on E.coli,Staphylococcus aureus,and Bacillus subtilis.

Key words: Leontopodium haplophylloides, phenethyl caffeate, dibutyl phthalate, anti-bacterial activities