Abstract: This research established an HPLC method for determination of ten kinds of polyphenol including the neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,caffeic acid,p-hydroxycinnamic acid,rutin,isoquercitrin,isochlorogenic acid B,isochlorogenic acid A and isochlorogenic acid C in the leaves of Ilex latifolia,and investigated the content difference and the antioxidant activity.Determination of 10 polyphenols in 50% methanol extract from the leaves of I.latifolia by HPLC and preliminary study on its antioxidant activity by DPPH.The results showed that Ten compounds were well separated with good linear correlations (r＞0.999 5).The average recoveries of ten compounds were 96.58%-112.03% and the RSD were less than 4% (n=6).The 50% methanol extract from the leaves of I.latifolia has good antioxidant activity.The HPLC method was rapid,accurate and stable,which could be used to detect simultaneously neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,caffeic acid,p-hydroxycinnamic acid,rutin,isoquercitrin,isochlorogenic acid B,isochlorogenic acid A and isochlorogenic acid C in the leaves of I.latifolia.There are certain differences in the contents of ten compounds between the samples from different producing areas and harvest times.The antioxidant capacity of 50% methanol extracts from the leaves of I.latifolia from different sources is significantly different.

Key words: Ilex latifolia, different areas, different harvest times, polyphenosl, HPLC, content determination, antioxidant activity