Abstract: To establish determination methods for content of 5 glycosides and total polysaccharides in Rehmanniae Radix and Rehmanniae Radix Praeparata,and compare the contents in the samples.HPLC was used to determine the contents of catalpol,rehmannioside D,leonuride,acteoside and isoacteoside,while UV spectrophotometry was used to determine the content of total polysaccharides.Compared with Rehmanniae Radix,the contents of catalpol,rehmannioside D,leonuride and acteoside were reduced at different degree in Rehmanniae Radix Praeparata,while the contents of isoacteoside and total polysaccharide were increased.The established determination method can be used to control the quality of Rehmanniae Radix and Rehmanniae Radix Praeparata.Different processing methods may have certain affection to the contents of glycosides and total polysaccharide in Rehmanniae Radix Praeparata.

Key words: Rehmanniae Radix, Rehmanniae Radix Praeparata, glycosides, total polysaccharide, content determination