Abstract: In order to evaluate the quality in Polygonum chinense var. chinense and Polygonum chinense var. hispidum from different varieties,the ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MSMS) method was used to evaluate the difference of chemical constituents on Polygonum chinense.The results showed that polyphenols and flavonoids were the main components of both extracts,and four compounds were identified as gallic acid,chlorogenic,ellagic acid and quercitrin based on the reference standards,and 21 compounds were identified or tentatively characterized in P.chinense according to the accurate m/z value analysis of TOF-MS data,and QTOF-MSMS fragment analysis and spectral data from references.This method with the characteristics of fast,sensitivity and efficient,could provide reference for further study in P.chinense,as well as provide scientific basis for the quality standarand research and quality assessment.

Key words: Polygonum chinense, UHPLC-QTOF-MSMS, chemical constituents