Abstract: To investigate eremophilane-type sesquiterpenoids from L.veitchiana,various chromatographic techniques,including silica gel column chromatography,ODS and preparative HPLC were used.The structures of the compounds were identified by means of spectroscopic and chemical data.As a result,13 eremophilane-type sesquiterpenoids were isolated from the 95% ethanol extract from Ligularia veitchiana and identified as eremophilenolide (1),eremophila-7(11),9-dien-8-one (2),eremophil-6-en-11-ol (3),8-oxo-eremophil-6-en-11-one (4),(6α,8α)-6-hydroxyeremophil-7(11)-en-12,8-olide (5),8β-hydroxyeremophil-7(11)-en-12,8α-olide (6),6β-hydroxy-8α-methoxyeremophil-7(11)-12,8β-olide (7),2α-hydroxyeremophil-11-en-9-one (8),6β-methoxy-8β-hydroxyeremophil-7(11)-en-12,8α-olide (9),6β-hydroxyeremophil-7(11)-en-12,8β-olide (10),6β-hydroxy-8β-methoxyeremophil-7(11)-12,8α-olide (11),6β,8β-dihydroxyeremophil-7(11)-en-12,8α-olide (12) and 6β,8α-dihydroxyeremophil-7(11)-en-12,8β-olide (13).Among them,compounds 5 and 10,7 and 11,12 and 13 are three pairs of diastereomers.Except for compound 3 and 5,all the above sesquiterpenoids were isolated from the plant for the first time.Cytotoxicities of all the compounds against cancer cell lines were evaluated in vitro by MTT assay,none of them showed significant growth inhibition of tumor cells HGC-27 and Caski.

Key words: L.veitchiana, eremophilane-type, sesquiterpenoid, anti-tumor