Abstract: The quality of Schisandrae Chinensis Fructus under different storage conditions was evaluated based on simultaneous determination of multiple bioactive constituents combined with multivariate statistical analysis.An analytical method based on UFLC-QTRAP-MS/MS was used for the simultaneous determination of fifteen components including lignans (schizantherin B,schisandrol B,schizandrin C,γ-schisandrin,deoxyschizandrin,schisantherin,schisandrin,schisanhenol,gomisin D,gomisin J,and angeloylgomisin H) and organic acids ((S)-malic acid,D(-)-tartaric acid,protocatechuic acid,and quinic acid) in Schisandrae Chinensis Fructus under different storage conditions (various package materials and temperatures).Samples from different storage conditions were evaluated synthetically by TOPSIS and gray related degree according to the content of 15 components.The results showed that fifteen components had good linearity (r > 0.999 1),and the limits of detection were all satisfactory.The average recoveries of standard addition for the compounds were between 96.64% and 99.96%,and the relative standard deviations were less than 5%.The greatest difference of r_i in grey related degree and C_i value in TOPSIS method were 57.5% and 81.3% respectively.The results of these two methods were similar and all showed that the holistic quality of No.4,No.3 and No.1 samples was better.The optimum storage condition for Schisandrae Chinensis Fructus was storing in a cool storage warehouse with a polyethylene sealing bag.These methods were useful for the overall assessment on quality of Schisandrae Chinensis Fructus,and this study may provide the foundation and support for optimizing suitable storage conditions of Schisandrae Chinensis Fructus.

Key words: Schisandrae Chinensis Fructus, storage conditions, UFLC-QTRAP-MS/MS, lignans and organic acids, simultaneous determination, grey related degree and TOPSIS