Abstract: To study the therapeutic effect of ethanol extract of processed Polygonum multiflorum and its main component emodin on non-alcoholic fatty liver disease (NAFLD) in zebrafish.A few wild-type zebrafish were randomly divided into control group,model group,processed Polygonum multiflorum extract group and emodin group.After 5 days of embryo fertilization,the control group was given with AP100.The model group was given with 2 mg/mL egg yolk powder (EY powder).The extract group was given 2 mg/mL egg yolk powder and a low dose group (1 mg/mL),a medium dose (1.5 mg/mL)and a high dose group (2 mg/mL).The emodin group was given 2 mg/mL egg yolk powder and a low dose group (0.25 μg/mL),a medium dose (0.5 μg/mL)and a high dose group (1 μg/mL).After 72h,the survival rate and BMI of zebrafish were observed.The incidence of fatty liver was evaluated by whole oil red staining,and the pathological morphology of liver tissue was observed by paraffin section.The content of zebrafish body fat was determined by triglyceride (TG) and total cholesterol (TC) kits.The alcohol extract of processed Polygonum multiflorum and emodin could significantly reduce the mortality,BMI,TG content of non-alcoholic fatty liver zebrafish and improve the liver lipid deposition in zebrafish juveniles.(P<0.05).It can be seen that the ethanol extract of processed Polygonum multiflorum and emodin can effectively treat zebrafish nonalcoholic fatty liver disease.And emodin may be the main active components in the treatment of NAFLD in processed Polygonum multiflorum.

Key words: processed Polygonum multiflorum, emodin, alcohol extract, nonalcoholic fatty liver disease, zebrafish