Abstract: In order to examine the absorbable components of the Bletilla striata effective parts in the intestine and their metabolic characteristics.Based on the intestine perfusion model,the collected intestinal perfusate,serum and bile of healthy SD rats were analyzed by UPLC-Q-TOF/MS.And combined with the reference substance,MS fragment information and the Single Mass Analysis function in the Masslynx V4.1 workstation,preliminarily speculated the structure of the absorption and metabolites.militarine,gymnoside I andα-isobutylmalic acid were preliminarily identified in serum and bile of rats.A total of gymnoside I and dihydrophenanthrene 5 metabolites were identified in rat circulating intestinal perfusion fluid,serum and bile.The method preliminarily explored the absorbable components and metabolic characteristics of Bletilla striata effective parts in the circulating intestinal perfusate of rats,and provided experimental basis for explaining the pharmacodynamic basis of white and medicinal materials.

Key words: Bletilla striata, effective part, intestinal perfusate, UPLC-Q-TOF/MS, serum, bile