Abstract: The aim of this research was to investigate the chemical constituents from Psidium guajavaLeaves and anticancer activity of selected compound.Thirteen compounds were obtained from the methanolic extracts of leaves of Psidium guajava by Prep.HPLC and column chromatography on silica gel,Sephadex LH-20,and MCI.On the basis of spectroscopic analysis,the isolated compounds were identified as 4,5-diepipsidial A (1),psidial A (2),guajadial (3),psiguadial A (4),psiguadial D (5),α-tocopherol (6),linoleic acid (7),quercetin-3-O-β-D-xylopyranoside (8),quercetin-3-O-α-L-arabinopyranoside (9),quercetin-3-O-α-L-arabinofuranoside (10),β-sitosterol (11),catechin (12),and gallic acid (13).Compound 1 was isolated from a natural source for the first time.Compound 5 was evaluated for their cytotoxicity in vitro against human tumor HL-60,SMMC-7721,A-549,MCF-7,and SW-480 cell lines and exhibited inhibitory effects on all five cells,with IC₅₀ values in the range of 4.08-11.23 μmol/L.

Key words: Psidium guajava, myrtaceae, chemical constituents, meroterpenoid, cytotoxic activity