Abstract: In order to investigate the diterpenoid alkaloid constituents of Aconitum karakolicum Rapaics,fifteen diterpenoid alkaloids were isolated by column chromatography over silica gel,RP-18,and HPLC.Their structures were identified as aconitine (1),3-deoxyaconitine (2),16-epipyroaconine (3),neoline (4),indaconitine (5),14-benzoyl-8-O-methylaconitine (6),spicatine A (7),15-α-hydroxyneoline (8),taurenine (9),14-benzoylaconine (10),14-benzoylaconine-8-oleate (11),lappaconitine (12),beiwudine (13),13-hydroxyfranchetine (14),and 8-O-linoleoyl-14-benzoylaconine (15) by extensive spectroscopic analyses and comparison of their spectroscopic data with these reported in the literature.Compounds 3-15 were isolated from the plants of A.karakolicum for the first time.In addition,the cytotoxicity and antifeedant activity of the isolates were evaluated by MTT and leaf-disk method,respectively.Compound 14-benzoylaconine-8-oleate (11) showed cytotoxicity against MCF-7,H460,and HepG2 human cancer cell lines,and the IC₅₀ values was 11.9,27.6 and 31.8 μM,respectively.The aconitine-type diterpenoid alkaloids,aconitine (1),3-deoxyaconitine (2),indaconitine (5),and beiwudine (13) showed considerably potent antifeedant activity (EC₅₀ < 2 mg/cm²).

Key words: Aconitum, Aconitum karakolicum Rapaics, diterpenoid alkaloid