To provide the scientific basis for the quality evaluation of Dysosma versipellis, its HPLC fingerprints was established,and the contents of five components were simultaneously determined.The analyses were performed on Thermo HyPURITY C₁₈ column (250 mm×4.6 mm,5 μm),and the mobile phase comprised of MeOH (A) - 0.4% phosphoric acid aqueous solution (B) with the gradient elution at a flow rate of 1.0 mL/min.The detection wavelength was set at 290 nm;the column temperature was maintained at 30 ℃ and the injection volume was 10 μL.The HPLC fingerprint with twenty-eight common peaks of D. versipellis in 16 batches of sample was established,and the similarities of samples were good.Eight components were identified by comparison with the reference substances.Five components,namely,(+)-tanegool-7′-methylether,4′-demethylpodophyllotoxin,quercetin,podophyllotoxin,kaempferol were analyzed quantitatively.The linear relationship of five components was good (r²≥0.999 0).The precision,repeatability and stability were all in line with quantitative requirements.The average recoveries (n= 6) of five components were between 98.7% and 99.6%,and the RSD were less than 3%.The established method is simple,stable and reliable,and can be expected for the quality control of D. versipellis.