Abstract: To optimize the extraction conditions of total flavonoids from seeds of Chenopoidium ambrosioides and evaluate its anti-tumor activity.Different polar organic solvents such as petroleum ether,ethyl acetate,n-butanol and distilled water were used as solvents alone or in sequence,7 extracts from seeds of C. ambrosioides were extracted by microwave-ultrasonic.The content of quercetin and kaempferol in 7 extracts were determined by HPLC.MTT method was used to evaluate their antitumor activity.Petroleum ether-ethyl acetate-n-butanol extract(S6) had the highest total flavonoid content of 96.39 mg/g among the 7 extracts.The HPLC results showed that the highest content of quercetin was 0.602 mg/g when ethyl acetate (S2) was used as the solvent.However,when petroleum ether-ethyl acetate-n-butanol (S6) was used as the extraction agent,the highest content of kaempferol was 0.479 mg/g.The MTT results showed 7 extracts had varying degrees of inhibitory effect on the proliferation of six kinds of cells (P< 0.05) and the lowest inhibitory effect on normal human liver cells L02.S6 had the best anti-tumor activity among them,and the IC₅₀ value of it on SMMC-7721 cells was 0.43 mg/mL,only 14.98% of the L02 cells;S6 can induce morphological changes and cytoskeletal reorganization of SMMC-7721 cells.In conclusion,extract(S6) using petroleum ether-ethyl acetate-n-butanol from seeds of Chenopoidium ambrosioides has the highest content of total flavonoids,the best solubility,and the strongest anti-tumor activity.

Key words: Chenopoidium ambrosioides, flavone, extraction condition, different solvents, anti-tumor