NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2020, Vol. 32 ›› Issue (2): 232-238. doi: 10.16333/j.1001-6880.2020.2.007

Special Issue: No.4

• Article • Previous Articles     Next Articles

Chemical constituents of Piper yunnanense

ZHANG Pan-hua1,YANG Jun1,LUO Ji-feng1,XIA Meng-yuan1,LONG Chun-lin2,WANG Li3*,WANG Yue-hu1*   

  1. 1Key Laboratory of Economic Plants and Biotechnology,and Yunnan Key Laboratory for Wild Plant Resource,Kunming Institute of Botany, Chinese Academy of Sciences,Kunming 650201, China;2Key Laboratory of Ethnomedicine (Minzu University of China),Ministry of Education,Beijing 100081,China; 3College of Horticulture and Landscape,Yunnan Agricultural University,Kunming 650201,China

  • Online:2020-02-29 Published:2020-04-23

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Abstract: Through column chromatography and semi-preparative high performance liquid chromatography (HPLC),10 compounds were isolated from the whole plants of Piper yunnanense.These compounds including four new amides,namely piperunnamides A-D (1-4),along with three known dipeptides,pipermullamides C-E（5-7）,and three known amides,(E)-N-(3,4,5-trimethoxycinnamoyl)pyrrolidine（8）,(E)-N-(5-methoxy-3,4-methylenedioxycinnamoyl)pyrrolidine（9）,and (Z)-N-(3,4,5-trimethoxycinnamoyl)pyrrolidine（10）.Their structures were elucidated by MS and NMR spectra.The cytotoxic activity against five tumor cell lines of these new compounds was evaluated by an MTS assay.The result showed that piperunnamide A (1) exhibited growth inhibitory activity against human leukemia HL-60 (IC50=10.99 μM) cell line,human liver cancer SMMC-7721 (IC50=17.10 μM) cell line,human lung cancer A-549 cell line (IC50=7.93 μM),human breast cancer MCF-7 cell line (IC50=16.63 μM),and human colon cancer SW480 cell line (IC50=9.16 μM).

Key words: Piperaceae, Piper yunnanense, amides, cytotoxicity

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