Abstract: To investigate the unknown constituents of Duhaldea nervosa by UHPLC-Q-Exactive Orbitrap MS combined with exclusion list technique.The analysis was performed on a Thermo Scientific Hypersil GOLD (100 mm×2.1 mm,1.9 μm),with a mobile phase consisting of 0.1% formic acid in water and acetonitrile for gradient elution.The flow rate was 0.3 mL/min and the injection volume was 2 μL.The data was acquired in negative mode at full scan and at the MS2 data triggered by data-dependent MS2 combined with exclusion list scanning.Finally,a total of 40 constituents was identified by comparing the reference compounds,bibliography data and database.To our best knowledge,it is the first time to report those compounds from Duhaldea nervosa.A rapid,effective,and accuracy method was built to study the constituents of Duhaldea nervosa in this study,which will be very helpful for the further study in quality control and material basis.

Key words: Duhaldea nervosa, UHPLC-Q-Exactive Orbitrap MS, identification, exclusion list