NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2020, Vol. 32 ›› Issue (5): 867-873. doi: 10.16333/j.1001-6880.2020.5.021

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Effect of taurine on triglyceride synthesis in HepG2 cells

MA Yu-xun1,SONG Qi1,ZHANG Jing1,2,GUO Jun-xia1,2, XIA Tian-ze2,CHEN Wen1,2*   

  1. 1Beijing Key Laboratory of Bioactive substances and Functional Foods,Beijing Union University,Beijing 100191,China; 2Food Science Department,College of Biochemical Engineering,Beijing Union University,Beijing 100023,China

  • Online:2020-05-28 Published:2020-06-16

Abstract:

The effect of taurine on triglyceride synthesis and its preliminary mechanism in oleic acid-treated HepG2 cells was researched in this article.A high triglyceride cell model was established by adding 0.05 mmol/L oleic acid to DMEM medium.HepG2 cells were treated with taurine at final concentration of 1,5,10 and 20 mmol/L for 24,48 and 72 h,and the levels of intracellular triglycerides were determined.The expression of SREBP-1c and the enzymes of AceCS1,ACC,FAS and ACSL1,associated with fatty acid synthesis,were detected by western blot after HepG2 cells were incubated with 5 mmol/L taurine for 24 h.Intracellular triglycerides levels were decreased by 1 mmol/L taurine treatment for 72 h (P<0.05),5 and 10 mmol/L taurine treatment for 24/48/72 h (P<0.05) and 20 mmol/L taurine treatment for 24/48 h (P<0.05) in high-triglyceride cells.And the protein expression of SREBP-1c,FAS,ACC,AceCS1 and ACSL1 were reduced while p-ACC was increased by 5 mmol/L taurine treatment for 24 h (P<0.05).Taurine inhibits the synthesis of fatty acid/triglyceride in oleic acid-treated HepG2 cells by regulating SREBP-1c and its downstream genes.

Key words: taurine, triglyceride, SREBP-1c

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