This study was to optimize the process of extracting total flavonoids and total saponins simultaneously from fibrous roots of Ophiopogon japonicas using response surface methodology. On the basis of single factor experiment,ethanol volume fraction,solid-liquid ratio,extraction time,and extraction times were selected as independent variables,the overall yield of flavonoids and saponins was used as the response value. Four factors and three levels tests were designed to optimize process conditions by the Box-Behnken experiment. Besides,the optimum macroporous resin was screened in the enrichment stage using adsorption and desorption effect as index. The results showed that the optimal extraction condition was ethanol concentration of 86%,solid-liquid ratio of 1∶15,extraction time of 1.4 h,and extracted three times. The extraction rate was 26.17%,and the total yield of flavonoids and saponins was 2.41% (The yield of total flavonoids and total saponins was 0.46% and 1.95%,respectively). The D101 resin revealed a good ability to enrich flavonoids and saponins. After enrichment,the recovery rate of total flavonoids and total saponins was 88.69% and 90.28%,the content of total flavonoids and total saponins was 7 times and 9 times higher than the crude extract,respectively. The process was simple and stable. It has important reference value for the development and utilization of the fibrous roots of O. japonicas as food resources.