In this paper,millet bran was used as raw material to extract millet bran protein with amylase.Four factors including enzyme dosage,time,temperature and pH value were investigated,and the extraction rate of millet bran protein was optimized by single factor test and response surface methodology.The best process for obtaining millet bran protein is as follows:enzyme dosage:1.8%,extraction time:2.2 h,extraction temperature:55.6℃,pH:6.5.Under these conditions,three tests were carried out and the average value was taken to obtain the best extraction rate of 48.53%.