To establish a method for content determination of multi-indicative components of Terminalia L. and provide a reference for the quality evaluation of Terminalia L..The content of 7 tannins were measured in 44 batches of different species of Terminalia L.and the analysis were conducted by variance analysis,cluster analysis (HCA),principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) to evaluate the differences in the tannis content among the three different species of Terminalia L..The results showed that there were distinct differences among 7 tannins content among the three species of Terminalia L..Variance analysis demonstrated that the differences of content were statistically significant (P<0.05).HCA,PCA and OPLS-DA suggested the differences among the species and the results analyzed by PCA and OPLS-DA indicated that the main different components were punicalagin A,punicalagin B,chebulinic acid,corilagin,chebulagic acid and gallic acid.The method is convenient and accurate,which provides a reliable analytical method and reference for the quality control of Terminalia L..