In this study,we selected zebrafish as experimental animals to study the biological effects on the organism and the protective activity and mechanism of polysaccharides from bamboo willow against kanamycin induced sensory hair cell injury by phenotype observation,fluorescence labeling and apoptosis detection.The results showed that when the concentration of polysaccharide was 30,40,50 μg/mL,it had no obvious toxicity or teratogenic effects after acting on zebrafish alone or with kanamycin.However,at the same concentration the polysaccharides could effectively reduce the accumulation of ROS in sensory hair cells,and inhibit the apoptosis of sensory hair cells.As a result,polysaccharides from bamboo willow played a protective effect on kanamycin-induced sensory hair cell damage.This study provided a basis for the application of polysaccharides in the development of drugs for the prevention and treatment of drug-induced deafness.