To explore the molecular mechanism of ovarian toxicity caused by tripolide,Chinese hamster ovary cells(CHO) were treated with triptolide.Then the gene expression was sequenced by HiSeq2000,and the biological functions of differentially expressed genes (DEGs) between treatment and control groups were analyzed with ClueGO and STRING 11.Compared with the control group,there were 740 DEGs in the treatment group,286 up-regulated genes involved in 16 GO biological processes while 454 down-regulated genes involved in 18 GO biological processes.And 22 KEGG signaling/metabolic pathways were enriched.Among them,several GO processes and KEGG pathways were closely related to the ovarian toxicity of triptolide,such as isoprenoid biosynthetic process,anatomical structure morphogenesis,regulation of signaling receptor activity,IL-17 signaling pathway,TNF signaling pathway,PI3K-Akt signaling pathway and steroid biosynthesis.The results showed that triptolide can disrupt various signaling pathways and metabolic processes of ovarian cells,which causes ovarian cells damage and bring out ovarian toxicity.