To study the chemical constituents of Justicia calycina (Nees).The 95% ethanol extract of Justicia calycina was separated and purified with commonly chromatographic methods (silica gel,Sephadex LH-20,ODS columns,and preparative HPLC).The structures of isolates were elucidated by extensive analysis of spectroscopic data (including IR,UV,HRESIMS and 1D NMR) and physicochemical properties.As a result,fifteen compounds were isolated from J. calycina and identified as lupeol (1),α-spinasterol (2),corchoionoside C (3),1-(4-hydroxy-3-methoxyphenyl)-2-［4-(3-hydroxypropyl)-2-methoxyphenoxy］-1,3-propane-diol (4),4-(2,3- dihydro-3-hydroxymethyl-5-(3-hydroxypropyl)-7-methoxybenzofuran-2-yl)-2-methoxyphenol (5),5-styrylfuran-2- carboxylic acid methyl ester (6),ferulyl aldehyde (7),(9E,12E)-methyl octadeca-9,12-dienoate (8),3,7,11,15- tetramethylhexadec-2-en-1-ol (9),α-linolenic acid (10),3,7,11,15-tetramethyl-n-octadac-13-en-3,4,6,7,8,11-hexol- 12-one (11),4-hydroxybenzoic acid (12),5-styrylfuran-2-carboxylic acid methyl ester (13),cinnamic acid (14) and 3,4-dihydroxy-benzoic acid (15).All of the above compounds were isolated from this plant for the first time.The results of antiviral assays indicated that none of the above compounds exhibit anti-RSV and anti-HSV-1 activities.The results of anti-inflammatory assay showed that compounds 1-7,14 show certain inhibitory activities on release of NO in RAW264.7 macrophages induced by LPS.