To screen the anti-inflammatory and anti-oxidative active fractions from fruits of Callicarpa longissima and study its chemical constituents.The activities of different fractions and compounds from fruits of C. longissima were evaluated by the inhibition of NO production and the multiple antioxidant methods including FRAP,ABTS and DPPH radical scavenging.The compounds were isolated and purified from the active fractions by using various chromatography techniques including silica gel,Sephadex LH-20,MCI,HPLC and recrystallization,and their structures were identified by spectroscopic methods.The results showed that the anti-inflammatory and anti-oxidative activities of the ethyl acetate fraction were stronger than others.Nine compounds,obtained from active fractions,were identified as two flavonoids:chrysosplenetin (1) and pachypodol (2);and seven diterpene phenols:carnosol (3),rosmanol (4),7-methoxyrosmanol (5),7-ethoxyrosmanol (6),carnosic acid-11-methylether (7),rosmadial (8),20-deoxocarnosol (9),respectively.Compounds 3,4,6 were the main compounds,and 1,2,5-9 were isolated from this plant for the first time.Compounds 1-9 have anti-inflammatory effects,among which compounds 3, 4 showed significant anti-inflammatory activity.