To study the terpenoids constituents and their hypoglycemic activities from Achillea alpina L..The extract was separated and purified by column chromatography on ODS,Sephadex LH-20 column chromatography and pre-HPLC.The structures of the compounds 1-13 were identified by NMR,MS and other spectroscopic techniques.Thirteen compounds have been isolated and purified from dichloromethane extract of A. alpina,and they were identified as ［3S-［3α,3aα,4α(Z),6α,6aα,9α,9aα,9bβ］］,2-butenoic acid,2-methyl-2,3,3a,4,5,6,6a,9,9a,9b-decahydro-6,6a,9-trihydroxy-3,6,9-trimethyl-2-oxoazuleno［4,5-b］furan-4-yl ester（1）,10β-hydroxyisodauc-6-en-14-al（2）,isodauc-6-ene-10β,14-diol（3）,aphanamol II（4）,ent-4(15)-eudesmene-1β,6α-diol（5）,12-hydroxy-α-cyperone (6),1β-hydroxy-α-cyperone (7),(E)-3,7,11-trimethyl-1,6,10-dodecatriene-3,5-diol (8),2,6,10-trimethyl-3,6,11-dodecatriene-2,8,10-triol (9),loliolide (10),8-hydroxyphellandral (11),(3R,6R,7E)-3-hydroxy-4,7-megastigmadien-9-one (12),saniculamoid D (13).Compounds 1-4,6,8 and 10-13 were isolated from the Achillea genus for the first time.Compounds 5,7 and 9 were isolated from this plant for the first time.Glucose consumption was analyzed to investigate the effect of compounds 1-13 in palmitic acid-mediated HepG2-insulin resistance cell.The result showed that compounds 5,9 and 10 (50 μmol/L) exhibited strong reversal IR activity.