The rapid analysis method for the chemical compositions in scammony methanol extract was established by UHPLC-Q-Orbitrap HRMS.The mass data were acquired by Full MS/dd-MS scan mode，and data analysis were performed by the information of accuracy molecular weight,fragment ion,summarized fragmentation pathway,combined with previous references and Compound Discovery(CD) software.Totally,92 compounds were identified from scammony methanol extract,including 72 resin glycosides,12 hydroxyl cinnamoyl quinic acids,eight ether insoluble resin glycoside acids.Among them,eight ether insoluble resin glycoside acids (compounds 10,12,13,16,17,18,19 and 20) and two tricaffeoylquinic acids (compounds 14 and 15) were detected first in scammony.The results provide a scientific basis for the future research on the improvement of the quality standard for scammony and its relative products.