The HPLC fingerprint of Epimeredi Indicae Herba was established to study its spectrum-effect relationship with antioxidant and hypoglycemic activities,so as to provide a basis for the quality evaluation of Epimeredi Indicae Herba.The fingerprints of ten batches of Epimeredi Indicae Herba were established by HPLC.The similarity evaluation system for chromatographic fingerprint of TCM (2012 edition) was used to evaluate the similarity evaluation.The common peaks were identified by comparison with the reference substance.SPSS 25.0 software was used for cluster analysis and principal component analysis.The antioxidant activity was evaluated by DPPH method and ABTS method,and the hypoglycemic activity was evaluated by pNPG method.Grey correlation analysis and bivariate correlation analysis were used to study the spectrum-effect relationship.The HPLC fingerprints of ten batches of samples were established,and the similarity was 0.767-1.000.Twenty-one common peaks were identified,and six components were identified.The contents of six components in ten batches of samples were significantly different.Ten batches of samples were clustered into three categories,and six main factors were obtained by principal component analysis.Ten batches of Epimeredi Indicae Herba had antioxidant and hypoglycemic activities in vitro.The grey correlation degree showed that the correlation degree of twenty-one common peaks with scavenging DPPH,ABTS⁺ free radicals and inhibiting α-glucosidase activity was greater than 0.731. Bivariate correlation analysis showed that peak 3 and peak 17 were significantly positively correlated with DPPH free radical scavenging activity,peak 1 and peak 4 were significantly positively correlated with ABTS⁺ free radical scavenging activity,peak 1,peak 4 and peak 11 (verbascoside) were significantly positively correlated with α-glucosidase inhibitory activity.This method can effectively analyze the quality of Epimeredi Indicae Herba from different producing areas,and provide a basis for the screening of antioxidation and hypoglycemic effective material basis and quality control of Epimeredi Indicae Herba.