The present study systematically analyzed the nutritional components of Yunshen (Codonopsis pilosula (Franch.) Nannf.var. volubilis (Nannf.)) based on national standard methods,and conducted acute oral toxicity tests in rats and three genetic toxicity tests to evaluate the food Safety of Yunshen.The results showed that the contents of protein,fat and polysaccharides in Yunshen were 9.190,3.700 mg/100 g,and 12.100%,respectively.The total content of amino acids reached 5.238 g/100 g,containing 21 mineral elements such as K and Ca.The highest content of K was 8 120.000 0 mg/kg; Four types of vitamins:vitamin A,vitamin C,vitamin E,and B vitamins were found,with B vitamins having the greatest content and a rich variety;Seven pesticide residues,including feniconazole,Jinggangmycin,tetraacetaldehyde,imidamide,thiafuramide,imidacloprid,and enoylmorpholine,as well as Salmonella and Staphylococcus aureus,were not detected in Yunshen;The median lethal dose (LD₅₀) of Yunshen was higher than 60.0 g/kg BW and it was classified into actually non-toxic according to acute toxicity dose;Three genotoxicity tests,including bacterial reverse mutation test,mammalian erythrocyte micronucleus test and mouse spermatogonium chromosome aberration test,were negative.Taken together, Yunshen was rich in protein,amino acids,mineral elements,and vitamins (especially B-group vitamins).Furthermore,pesticide residues and microbial indicators met the requirements of China′s national food safety standards,and no significant toxic effects were observed under the conditions of this experiment.