Bee pollen polyphenols are a class of natural substances that can effectively lower blood sugar. α-Amylase is a key enzyme for blood glucose control in higher organisms,and inhibiting its activity can help control postprandial blood glucose.In this study,buckwheat bee pollen polyphenol (BBPP) was used as the sample to investigate the inhibition effect of BBPP on the activity of α-amylase by UV-vis and fluorescence spectrometry.The results showed that BBPP had a strong inhibitory effect on α-amylase,with a half maximal inhibitory concentration (IC₅₀) of 2.00 ± 0.06 mg/mL,and a reversible mixed inhibition type.Moreover,all the treatments including high temperature,strong alkali,light and high concentration of oxidants and reducing agents could decrease the inhibition effect of BBPP on α-amylase.At the temperature of 273-298 K and 298-310 K,the binding process was dynamic quenching and static quenching,respectively.As the concentration of BBPP increased,the maximum emission wavelength of α-amylase fluorescence had a blue shift,indicating that the conformation of α-amylase altered and the hydrophobicity increased.In conclusion,BBPP has a strong inhibitory effect on α-amylase,which has important theoretical reference value for clinical development and utilization of buckwheat bee pollen polyphenol.