NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2024, Vol. 36 ›› Issue (6): 993-1003. doi: 10.16333/j.1001-6880.2024.6.010

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Multi-index composition analysis of different processed products of Astragali Radix based on HPLC fingerprint combined with chemical pattern recognition method

ZHOU Jing-jing1,ZHOU Jie1,ZHANG Hong-wei1,DANG Wen-fei1,DOU Xia2,3,JIN Zi-ming2,3*   

  1. 1Gansu University of Traditional Chinese Medicine;2Affiliated Hospital of Gansu University of Traditional Chinese Medicine; 3Yang Xicang National Famous Old Chinese Medicine Expert Studio,Lanzhou 730030,China
  • Online:2024-06-26 Published:2024-06-26

Abstract:

This study provides theoretical support for the comprehensive utilization of Astragali Radix processed products by establishing HPLC fingerprints and multi-component content determination methods for different processed products of Astragali Radix,and combining them with chemometric methods for comparative analysis.The fingerprints of Astragali Radix processed products were established by HPLC and the contents of six components were determined,and evaluate the differences of processed products of Astragali Radix by hierarchical clustering analysis (HCA),principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) to find the differential components.The results showed that a total of 20 common peaks were obtained from different processed products (ten batches each),among which six common peaks were identified,and the linearity of the six components was good in their respective mass concentration ranges (r>0999),with average spiked recoveries ranging from 100.5% to 102.1%,and the relative standard deviation (RSD) was 0.77%-2.1%;The similarity results of fingerprint mapping showed that the similarity of ten batches each of honey bran-processed Astragali Radix,honey-processed Astragali Radix,salt-processed Astragali Radix,and wine-processed Astragali Radix was above 0.900,but the similarity of different types of concoctions decreased;Hierarchical clustering analysis can cluster the four kinds of processed products into three categories,the results of principal component analysis and cluster analysis are consistent and can be corroborated with each other,orthogonal partial least squares-discriminant analysis can effectively differentiate the four kinds of processed products,and nine main difference components were screened based on the variable importance of projection (VIP) greater than 1,calycosin-7-glucoside,ononin,and 5-hydroxymethylfurfural were identified as the major landmark components causing compositional differences among different processed products of Astragali Radix.The HPLC fingerprint combined with stoichiometry of Astragali Radix processed products established by this institute is accurate and reliable,which can provide a reference for the quality control and quality evaluation of Astragali Radix processed products.

Key words: Astragali Radix, HPLC, fingerprint, content determination, chemical pattern recognition, quality evaluation

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