Abstract:

This study aims to investigate the correlation between the external color,microscopic characteristic color and the pharmacodynamic component content of Lycii Fructus. Based on 30 batches of Lycii Fructus,the external color value was determined by color difference method,the microscopic characteristic color information was determined by microscopic imaging technology,the pharmacodynamic component content was determined by ultraviolet-visible spectrophotometry method,and HPLC method.Pearson correlation analysis was used to analyze the correlation between external color,microscopic characteristic color and the pharmacodynamic component content of Lycii Fructus. There was a significant negative correlation between the L^* value of epidermal cells in outer pericarp and the L^*,a^*,b^*,E^*_ab values of color of Lycii Fructus (P<0.05).The values of L^* in mesocarp parenchyma cells were negatively correlated with the values of L^*,a^*,b^*,E^*_ab,and were significantly correlated with the values of b^*,E^*_ab (P < 0.05).The b^* value of mesocarp parenchyma cell was positively correlated with L^*,a^*,b^*,E^*_ab values,and significantly correlated with a^*, b^*,E^*_ab values (P<0.05).The E^*_ab values of mesocarp parenchyma cells were negatively correlated with the a^*,b^* and E^*_ab values of the color of Lycii Fructus,and were significantly correlated with the a^* and b^* values (P<0.05).Chlorogenic acid content was negatively correlated with a^* and E^*_ab values of Lycii Fructus.Ferulic acid content was positively correlated with a^* and E^*_ab values,and significantly correlated with a^* values (P<0.05).There was a low negative correlation between quercetin content and L^* value of Lycii Fructus.The content of Lycii Fructus polysaccharide was positively correlated with the a^* value of exocarp epidermal cells.The content of betaine was negatively correlated with the b^* value of seed stone cells.Chlorogenic acid content was negatively correlated with b^* value in mesocarp parenchyma cells.Ferulic acid content was negatively correlated with L^* and E^*_ab values in exocarp epidermis cells,positively correlated with b^* values in exocarp epidermis cells,and significantly correlated with L^* and b^* values (P<0.01).There is a certain correlation between the external color and the microscopic characteristic color and the pharmacodynamic component content of Lycii Fructus,which provides a scientific basis for the quality evaluation of Lycii Fructus decoction pieces.

Key words: Lycii Fructus, color difference method, color of Lycii Fructus, microscopical characteristic color, pharmacodynamic component