Abstract:

This study aims to investigate the sesquiterpenoids from the root of Chloranthus fortunei and their anti-inflammatory activity. By using silica gel column, ODS column, Sephadex LH-20 gel column and semi-preparative HPLC, the 95% ethanol extract of the root of C. fortunei was isolated and purified. The structures of isolated compounds were elucidated by NMR, HR-ESI-MS, and ECD methods. Seven sesquiterpene dimer compounds were obtained from the root of C. fortunei, including chlorantuforlide A (1), chloramultilide C(2), chlorahololide D (3), chlorahololide B (4), fortulactone A (5), shizukaol C (6), and fortunilide C (7). Compound 1 was a new lindenane-type sesquiterpene dimer, and compounds 2-4 and 6 were isolated from C. fortunei for the first time. Activity experiment showed that compound 1 could significantly inhibit the production of NO in RAW 264.7 cells induced by lipopolysaccharide, showing potent anti-inflammatory activity.

Key words:

Chloranthus fortunei, sesquiterpene dimer, anti-inflammatory activity