To investigate the protective effect of Phellodendri Chinensis Cortex-Vaccariae Semen (PCC-VS) on inflammatory injury of chronic non-bacterial prostatitis(CNP).In this study,41 active ingredients and 156 corresponding target proteins were screened by TCMSP and literature search.OMIM and GeneCards databases were used to screen out 9 848 disease targets related to CNP Cystoscape and String software were used to construct protein target network interaction map and active ingredient-disease target-pathway.PPI results mainly involved key proteins such as interleukin -6(IL-6),tumour necrosis factor-α (TNF-α),interleukin-1 beta (IL-1β),tumor protein-53 (TP53) and RAC-alpha serine/threonine-protein kinase (AKT1).GO and KEGG pathway enrichment analysis were conducted using the Metascape database,enrichment of KEGG pathway involves PI3K-AKT,lipid and atherosclerosis,prostate cancer,MAPK signaling pathways;Molecular docking of active drug components and core targets was performed;In order to verify the above data,the serum levels of IL-1β,interleukin-18(IL-18),TNF-α in rats were detected in animal experiments.The pathological changes of prostate tissue were observed by HE staining.The core target proteins IL-6,TNF-α,IL-1β and the related proteins in the screened pathway were detected by Western blot.Animal experiments showed that the regulation of inflammatory factors and the expression of inflammation-related proteins were inhibited in each treatment group.These results suggest that the PI3K-AKT signaling pathway may be involved in the improvement of CNP through multi-component and multi-target pathways.