Abstract: To compare the intestinal absorption characteristics of Laportea bulbifera extract in AA and normal rats.This experiment adopts the systemic intestinal perfusion method,set up big rats model of rheumatoid arthritis,application of UPLCMS/MS detection of intestinal perfusion fluid in neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,rutin,isoquercetin,quercetin,kaempferol-3-O-rutinoside and galuteolin content,explore various components in the different factors (pHvalue,the concentration of the drug,the intestine,bile and Pgp inhibitors) under the intestinal absorption characteristics.The results showed that the absorption mode of quercetin was active transport,and the absorption mode of the other 7 components was passive diffusion.Except chlorogenic acid and cryptochlorogenic acid,the best absorption of each component was in ileum under normal condition and duodenum under pathological condition,so it was speculated that the specific sites of drug absorption would be affected by the disease.The absorption of each component is affected by pH,bile and pgp,among which quercetin may be the substrate of pgp.Rheumatoid arthritis can affect the intestinal absorption of Laportea bulbifera extract in rats,and its mechanism needs to be further studied.

Key words: Laportea bulbifera extract, in situ intestinal circulation perfusion model;rheumatoid arthritis rats;intestinal absorption characteristics