Abstract: This paper analyzed the status,problem and countermeasure of the application of tissuecultured technology on the conservation and exploration of medicinal plants used Dendrobium huoshanense C.Z.Tang et S.J.Cheng as an example.Although the plant tissue culture technique is widely used in the conservation and utilization of rare and endangered medicinal plants,it still has bottlenecks such as how to make tissue culture stocks obtain the ability to produce similar bioactive components as their wild correspondences.The previous phytochemical investigation revealed that remarkable differences in both contents and composition of active components are existed between wild D.huoshanense and testtube plantlet tamed stock.This caused the lower quality of testtube plantlet tamed D.huoshanense and became a of the germplasm resource conservation and utilization of the endangered medicinal plant.The removing of the endophytic fungi in the sterile stage of testtube seeding might cause the absence of the endophyteoriginated metabolites in the plants.Reintroducing endophytes in to the tissuecultured stocks and thus recovering the ability of producing the lacked chemicals,or obtaining seedlings induced from the seeds,might be a tactics.

Key words: Dendrobium huoshanense, modern biotechnology, rare and endangered medicinal plants, conservation of resource