NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2010, Vol. 23 ›› Issue (6): 1069-1072.

• Article • Previous Articles     Next Articles

Histochemical Localization of Flavonoids and Determination of Baicalin in Regenerated Plants of Scutellaria amoena C. H. Wright

CEN Ju-ren1,2,BU Huai-yu1*,KONG Xiang-he1,WANG Ying-juan1,JIA Jing-fen1   

  1. 1Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, Xi'an 710069, China; 2 School of Life Sciences, Bejing Normal University, Beijing 10075, China
  • Received:2009-03-25 Online:2011-01-10 Published:2011-10-19

Abstract:

The histochemical method was applied in the study of the localization of flavonoids in the regenerated plantlets of Scutellaria amoena C. H.Wright. The results showed that the flavonoids were mainly distributed in the epidermis and cortex parenchyma of the vegetative organs. The flavonoids were also found to accumulate in the tentacles of the stems and leaves. In addition, the content of baicalin in the regenerated plantlets was determined by HPLC. The content of baicalin in the stems and leaves (0.43%) was higher than that in the roots (0.12%).

Key words: Scutellaria amoena C. H. Wright, regenerated plantlet, histochemistry, flavonoids, baicalin, HPLC