NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2010, Vol. 23 ›› Issue (6): 1073-1076.

• Article • Previous Articles     Next Articles

Determination of 4-Hydroxyacetophenone and 2,4-Dihydroxyacetophenone in Cynanchum atratum Bge. by HPLC

WANG Yuan-shu，ZHAO Xin-chao，BAI Hong*，LIU Yong-jun，LIU Ai-qin   

  1. Institute of Materia Medica, Shandong Academy of Medical Sciences, Key Laboratory for Modern Medicine and Technology of Shandong Province, Jinan 250062, China

  • Received:2009-06-19 Online:2011-01-10 Published:2011-10-19

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Abstract:

An HPLC method was established for assays of 4-hydroxyacetophenone and 2,4-dihydroxyacetophenone in Cynanchum atratum Bge.. Analysis was carried out on ZORBAX SB-C18 (4.6 mm ? 250 mm, 5 mm) column with methanol-0.1% acetic acid (35:65), at flow rate of 1.0 mL/min. The detection wave length was 275 nm. Linear correlations of 4-hydroxyacetophenone and 2,4-dihydroxyacetophenone were good in the ranges of 1.04-104.00 ?g/mL (r=0.9999) and 0.25-25.00 ?g/mL (r=0.9999). The average recoveries were 95.40% and 93.28%, and RSD were 3.71% and 1.63%, respectively. This method was simple, accurate, reproducible, and suitable for the determination of C. atratum Bge.

Key words: HPLC, Cynanchum atratum Bge., 4-hydroxyacetophenone, 2,4-dihydroxyacetophenone, determination