NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2011, Vol. 23 ›› Issue (1): 15-19.

• Article • Previous Articles     Next Articles

Separation of Conjugated Linoleic Acid Isomers in Milk by Micellar Electrokinetic Chromatography

WANG La-mei, CHEN Yan, LIU Xiao-hua, CAO Yu-sheng*   

  1. State Key Laboratory of Food Science and Technology, Sino-German Joint Research Institute, Nanchang University, Nanchang 330047, China

  • Received:2010-04-02 Published:2011-07-20

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Abstract:

A micellar electrokinetic chromatography method was used to analyze the major conjugated linoleic acid (CLA) isomers in milk. Under the optimized conditions (80 mM borate (pH 9.0), 54 mM sodium dodecyl sulphate, 4% (w/v) β-cyclodextrin, 8 M urea, 4%(v/v) ethanol, 25 kV and 20 ℃), the major CLA isomers 9c,11t-CLA and 10t,12c-CLA could be separated within 15 min by MEKC. Limit of detection of this method is 0.081 ng/mL. The results indicated that there was a significant difference in the total CLA content among raw milks, pasteurized milk, UHT milk (P<0.001). However, the content of 10t, 12c-CLA from all samples was similar (3 %). The result also showed there was a significant difference in the total CLA content and 10t, 12c -CLA content among the cow milk, goat milk and buffalo milk.

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