Physicochemical Properties of a Water-soluble Polysaccharide Fraction SHP-3 Purified from Soybean Hull

Abstract

Abstract:

The water-soluble polysaccharide of soybean hull (SHP) was extracted by hot water and three fractions were obtained by using DEAE-cellulose anion exchange column. The physicochemical properties of the purified fractions SHP-3, eluted with 0.3 M NaOH, were investigated. Gel permeation chromatography showed that the molecule weight of SHP-3 was 45554. GC analysis of the monosaccharide composition showed that the molar ratio of Rah: Fuc: Ara: Xyl: Man: Gal: Glu was 3.55:0.44:11.58:1:7.45:5.12:1.12 and the content of galacturonic acid was 26.71% (wt.%). The ultraviolet spectra indicated that there was no presence of protein in SHP-3. The molar percentages of (1→2) glycosidic linkage, (1→4) glycosidic linkage and (1→6) glycosidic linkage were 11.4%, 68.9% and 19.7%, respectively.

Key words: soybean hull, water-soluble polysaccharide, purification, monosaccharide composition, GC

LAI Fu-Rao, WU Hui, LI Xiao-Feng, REN Yao, WU Lei-Yan, WEN Qi-Biao. Physicochemical Properties of a Water-soluble Polysaccharide Fraction SHP-3 Purified from Soybean Hull[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2011, 23(1): 76-81.

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