Biotransformation and Biosynthesis of Saikosaponins in Adventitious Root System of Bupleurum chinese

Abstract

Abstract:

The conditions and methods of using Bupleurum chinese adventitious root system to produce saikosaponins were studied through adding precursors，accelerators and high sucrose concentrations. The results showed that the addition of proper concentrations of magnesium sulfate，potassium nitrate，sodium pyruvate and sucrose in medium had the effects on biotransformation and biosynthesis of saikosaponins. The most suitable components were 1/2 MS medium adding 0.37 g/L magnesium sulfate，5.7 g/L potassium nitrate，2.0 mmol/L sodium pyruvate and 50 g/L sucrose，in which the saikosaponins were enhanced 9.74 times.

Key words: Bupleurum chinese DC, adventitious root, biotransformation, saikosaponins

GUAN Qian, HAO Jian-Ping, YANG Dong-Fang, HAN Dan, GAO Ke-Qing, ZHOU Qiong. Biotransformation and Biosynthesis of Saikosaponins in Adventitious Root System of Bupleurum chinese[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2011, 23(2): 208-211.

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Biotransformation and Biosynthesis of Saikosaponins in Adventitious Root System of Bupleurum chinese

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