Optimizing Hydrolysis Conditions of β-Lactoglobulin with Trypsin to Obtain the ACE Inhibitory Peptide

Abstract

Abstract:

Three-factor quadratic orthogonal rotation design and in vitro assay was adopted to optimize the hydrolysis conditions of β-lactoglobulin with trypsin for obtaining the Angiotensin-I-converting enzyme (ACE) inhibitory peptide. The results showed that the regression equation of the impacts of the substrate concentration (X₁)，temperature (X₂)，enzyme，and substrate mass ratio (X₃) on the ACE inhibition rate was Y = 50.62–2.33X₁–1.97X₂+5.81 X₃–3. 36X₂X₃–6.56X₂₂–1.96X₃₂. The optimum conditions were as following: when substrate concentration was at 60 g/L，the hydrolysis temperature at 30 ℃，enzyme and substrate mass ratio at 5.5%，the hydrolysis time in 6 h，the hydrolysis product of ACE inhibitory activity was on the maximal inhibition rate of 53.86%.

Key words: β-lactoglobulin, trypsin, ACE

MEI Lin, WANG Zhi-Geng, WANG Zi-Long, XUE Xiu-Heng. Optimizing Hydrolysis Conditions of β-Lactoglobulin with Trypsin to Obtain the ACE Inhibitory Peptide[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2011, 23(2): 295-298.

References

1 Cao WH(曹文红),Zhang CH(章超桦).Antihypertensive peptides derived from food proteins and enzymatic approaches to their production(Ⅰ).Food Sci Technol(食品科技),2002,(4):9-10.
2 Liu ZG(刘志国),Chen JY(陈江源).Preparation of ace inhibitor from enzyme-degested zein.Food Res Dev(食品研究与开发),2006,27(2):138-141.
3 Fahmi A,Morimura S,Guo HC,et al.Production of angiotensin I converting enzyme inhibitory peptides from sea bream scales.Process Bichem,2004,39:1195-1200.
4 He HL(何海伦),Chen XL(陈秀兰),Sun CY(孙彩云),et al.Research progress in inhibitory peptides of angiotensin converting enzyme.China Biotechnol(中国生物工程杂志),2004,24(9):7-11.
5 Cushman DW,Cheung HS.Spectrophotometric assay and properties of the Angiotensin-coverting enzyme of rabbit lung.Biochem Pharmacol,1971,20:1637-1648.
6 Leclerc PL,Gauthier SF,Bachelard H,et al.Antihypertensive activity of casein-enriched milk fermented by Lactobacillus helveticus.Intern Dairy J,2002,12:995-1004.
7 Meano M,Yamamoto N,Takano T.Identification of an antihypertensive peptide from casein hydrolysate produced by a proternase from lactobacillus helveticus CP790.J Dairy Sci,1996,79:1316-1321.
8 Li GH,Le GW,Shi YH,et al.Angiotensin-I-converting enzyme inhibitory peptides derived from food proteins and their physiological and pharmacological effects.Nutr Res,2004,24:469-486.
9 Miyaguchi Y,Sakai K,Yonekure M,et al.Some properties of succinglated globin.Nippon Shokunin Kogoy Gakkaishi,1989,36:720-725.
10 FitzGerald RJ,Meisel H.Milk protein—derived peptide inhibitors of angiotensin—Iconverting enzyme.Br J Nutr,2000,84:33-37.
11 Guo SG,Zhao MM,Cui C,et a1.Preparation anantioxidant properties of decolorized porcine hemoglobin hydrolysates.Chem Bioeng,2007,24(4):49-53.
12 Chang C,Wu KC,Chiang SH.Antioxidant properties and protein compositions of porcine hemoglobinhydrolysates.Food Chem,2007,100:l537- l543.

[1]	GUI Ming-an, DE Lan, YANG Gui-bin, GAO Dan, YANG Zhuo-ma, ZHUOMA Dong-zhi, YUAN Rui-ying. Optimization of flash extraction process and in vitro antioxidant and anti-inflammatory activities of total iridoid glycosides from the Tibetan medicine Lagotis yunnanensis [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2024, 36(增刊1): 91-97.
[2]	YAN Wen-si, ZHANG Yan, GU Pei-shan, ZENG Zi-qian, HE Xin, SU Xiang-dong, XUE Yong-bo. Chemical constituents from Fallopia cynanchoides (Hemsl.) Harald.var. cynanchoides [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2024, 36(9): 1519-1527.
[3]	LI Min, CHI Jun, ZHANG Wei-jin, WANG Zhi-min, DAI Li-ping. Study on the chemical constituents from ethyl acetate extract of Ipomoea turbinata seeds [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2024, 36(9): 1528-1536.
[4]	ZHANG Ya-mei, HAN Hong-ping, SONG Dao-guang. Optimization of the extraction process of Fritillaria unibracteata fluid extract by response surface methodology and its quality evaluation [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2024, 36(9): 1554-1563.
[5]	HU Di-di, XUE Hao, JIN Xu-dong, LI Wan-yu, XU Xiang-di, CHEN Jing. Optimization of the preparation technics of artemisinin-loaded lactic-co-glycolic acid nanoparticles based on Box-Benhnken response surface method combined with a single factor analysis [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2024, 36(9): 1592-1599.
[6]	XIE An-qi, CHI Jun, ZHANG Wei-jin, WANG Zhi-min, DAI Li-ping. Chemical constituents from EtOAc extract of Pinelliae Rhizoma and their anti-inflammatory activity [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2024, 36(8): 1339-1349.
[7]	JIANG Li-jie, WEN Le-le, SHI Jia-yi, DENG Ya-hui, LIU Ju-zhao, YUAN Qiang, CUI Qi. Screening and optimization of essential oil extraction process model of Citrus × aurantium Changshan-huyou pericarp and its chemical constituents and antioxidant activity [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2024, 36(7): 1101-1111.
[8]	ZHANG Kai-yue, ZHOU Yong-shun, HAN Kai, WANG Lu, CHEN Fu-long, GAO Jian-feng. Extraction optimization and purification of intracellular acidic polysaccharides from Chlorella sp. GTD4C-2 and their antioxidant activity [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2024, 36(7): 1112-1120.
[9]	ZHANG Yi-ning, YU Zhi-qi, QU Shi-xu, LIU Tong-ge, ZHANGYu-hong. Optimization of the extraction process of cannabidiol from hemp leaves by response surface methodology and its content distribution in different parts [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2024, 36(3): 369-376.
[10]	WANG Xiao-lan, SHIJing-ya, LI Meng, WANG Ying-qing, ZHENG Xiao-ke, FENG Wei-sheng. Protective effect of polyacetylene compounds from the stem and leaves of Chrysanthemum morifolium Ramat on the HUVECs damage induced by ox-LDL [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2024, 36(10): 1742-1750.
[11]	YANG Dan-dan, ZHU Yu-chang, ZHOU Da-zhai. Optimization of alkali extraction process of polysaccharides from Premna puberula Pamp leaves by response surface methodology and its bioactivity [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2024, 36(1): 94-101.
[12]	PAN Bo-ya, MENG Xian-qun, KANG Ting-guo, YIN Hai-bo, XU Liang, WANG Dan. Optimization of extraction process of Arctii Fructus polysaccharide based on response surface method and its anti-inflammatory effect on cells [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2024, 36(1): 102-108.
[13]	DING Lin-ling, HU Jiang-miao, LIU Hong-jun, BAO Yu, LI Xiao-jue, HUA Yan. Optimization of ultrasound-assisted enzymatic extraction of lacquer wax and its physicochemical property [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2023, 35(增刊2): 84-92.
[14]	CAO Wen-ying, XU Bing-yan, PENG Guo-qing, CHEN Yin, HUANG Jian-ping, HUANG Sheng-xiong. Research progress of secondary metabolites based on Astragalus membranaceus hairy root technique#br# #br# [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2023, 35(增刊2): 164-173.
[15]	DING Jin, TIE Fang-fang, HU Na, WANG Hong-lun, DONG Qi. Study on optimization of the extraction process of total alkaloids in Anisodus tanguticus (Maxim.) Pascher by response surface methodology and its anti-inflammatory activity [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2023, 35(增刊1): 73-79.