Optimizing the Extraction Process of Four Important Alkaloids from Catharanthus roseu by Ultrasonic Method

Abstract

Abstract:

In order to explore the efficient and low contaminated extraction process of vindoline，catharanthine，vincristine and vinblastine from Catharanthus roseus，comprehensive optimization of condition in ultrasonic-methanol method were studied. On the basis of single factor experiments，orthogonal test was made，and the optimum parameters for obtaining vindoline and catharanthine were determined as follows: Methanol as solvent was used for 180 min，which pH value was 4.0. The ratio of raw material to solvent is 1:15 (w/v)，the extraction temperature is 45 ℃，and extraction frequency is 59 KHz. The yield of vindoline and catharanthine in the conditions were 0.164% and 0.081%, respectively.

Key words: ultrasonic-methanol method, orthogonal experiment, Catharanthus roseus, alkaloid, extraction process, optimization

ZHANG Jing, LONG Xiao-Hua, LIU Ling, LIU Zhao-Pu. Optimizing the Extraction Process of Four Important Alkaloids from Catharanthus roseu by Ultrasonic Method[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2011, 23(2): 309-313.

[1]	PAN Yuan, TAN Jun, ZHAO Xiao, LYU Hui, YANG Shi-chun, CHEN Da-xia. Transcriptome analysis of biosynthesis of flavonoid and alkaloid from Citrus aurantium L. [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2024, 36(增刊1): 1-12.
[2]	LI Min, CHI Jun, ZHANG Wei-jin, WANG Zhi-min, DAI Li-ping. Study on the chemical constituents from ethyl acetate extract of Ipomoea turbinata seeds [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2024, 36(9): 1528-1536.
[3]	ZHANG Ya-mei, HAN Hong-ping, SONG Dao-guang. Optimization of the extraction process of Fritillaria unibracteata fluid extract by response surface methodology and its quality evaluation [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2024, 36(9): 1554-1563.
[4]	JIANG Li-jie, WEN Le-le, SHI Jia-yi, DENG Ya-hui, LIU Ju-zhao, YUAN Qiang, CUI Qi. Screening and optimization of essential oil extraction process model of Citrus × aurantium Changshan-huyou pericarp and its chemical constituents and antioxidant activity [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2024, 36(7): 1101-1111.
[5]	ZHANG Kai-yue, ZHOU Yong-shun, HAN Kai, WANG Lu, CHEN Fu-long, GAO Jian-feng. Extraction optimization and purification of intracellular acidic polysaccharides from Chlorella sp. GTD4C-2 and their antioxidant activity [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2024, 36(7): 1112-1120.
[6]	ZHANG Hong-qi1, 2, ZHU Lie-qin1, ZENG Ya-ni1, CUI Min1, ZHOU Ying-xia1, DU Juan3. Optimization of extraction process of Phellinus lonicerinus polysaccharide and analysis of its antioxidant activity in vitro [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2024, 36(4): 581-588.
[7]	DENG Chao-fan, JIANG Zhi-bo, MA Xiao-li, NAN Ze-dong. Research progress on benzylisoquinoline components from genus Corydalis [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2024, 36(4): 694-716.
[8]	LEI Yu-jie, CHENG Jing-jing, TANG Cheng, MENG Yan. Optimization of extraction process of chitin from Periostracum Cicadae and evaluation of antioxidant activity and hemostatic effect [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2024, 36(2): 268-278.
[9]	ZHENG Peng, HUANG Min, ZHANG Jin-lian, ZHENG Yu-qing, SHANG Jie, GUO Jing-ying, LIU Ming-gui, XU Cong-long. Study on the chemical composition of Jiangxi specialty Chinese medicine Chaxiong based on UPLC-Q-TOF-MS/MS [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2024, 36(1): 52-62.
[10]	YANG Dan-dan, ZHU Yu-chang, ZHOU Da-zhai. Optimization of alkali extraction process of polysaccharides from Premna puberula Pamp leaves by response surface methodology and its bioactivity [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2024, 36(1): 94-101.
[11]	PAN Bo-ya, MENG Xian-qun, KANG Ting-guo, YIN Hai-bo, XU Liang, WANG Dan. Optimization of extraction process of Arctii Fructus polysaccharide based on response surface method and its anti-inflammatory effect on cells [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2024, 36(1): 102-108.
[12]	DING Jin, TIE Fang-fang, HU Na, WANG Hong-lun, DONG Qi. Study on optimization of the extraction process of total alkaloids in Anisodus tanguticus (Maxim.) Pascher by response surface methodology and its anti-inflammatory activity [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2023, 35(增刊1): 73-79.
[13]	WU Jun-ying, ZHANG Wu-gang, LANG Yi-fan, HUANG Zhi-chao, YANG Wu-liang, YANG Jie , CHEN Hai-fang. Chemical composition analysis of mango kernels by UPLC-Q-TOF-MS/MS [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2023, 35(6): 949-965.
[14]	LIU Ju-zhao, XIAN Meng-xue, KONG Wei-hua, TIAN Xiao-li, YUAN Qiang, CUI Qi. Optimization of extraction process and composition analysis of essential oil from Amomum tsao-ko [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2023, 35(5): 766-780.
[15]	WANG Hao, LIU Yan-fang, WANG Wei, RUI Hai-yun, DONG Hao-yan. Alkaloids from leaves of Nelumbo nucifera Gaertn. and their agonistic activities on 5-HT_2A and 5-HT_2C receptors [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2023, 35(4): 656-661.

Optimizing the Extraction Process of Four Important Alkaloids from Catharanthus roseu by Ultrasonic Method

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments