Preventive Effect of Different ω-3/ω-6 Constituent Ratio of Compatibility Safflower Seed Oil on SH-SY5Y Cells against Oxidative Cell Damage

Abstract

Abstract:

In order to investigate the preventive effect of different ω-3/ω-6 constituent ratio of compatibility safflower seed oil (CSSO) on SH-SY5Y human neuroblastoma cells against oxidative cell damage, an oxidative cell damage model was established by using hydrogen peroxide (H₂O₂) as a donor of free reactive oxygen species, and after treatment of cells with different ω-3/ω-6 constituent ratios of CSSO, the alteration of cell viability and cellular apoptosis were determined by MTT (methyl thiazolyl tetrazolium) assay and flow cytometry. We successfully established the oxidative cell damage model of SH-SY5Y cells induced by H₂O₂, with the IC₅₀ value of 1089.54 μmol/L H₂O₂. The preventive effect of CSSO against oxidative cell damage increased along with the decreasing amount of ω-3 content in CSSO. Moreover, at the ω-3/ω-6 ratio of 1:6.68 and effective dose range of 375~750 μg/ml CSSO, the cell viability was increased significantly to a higher level in the treatment group (84.1%) than in the control (61.1%), and cellular apoptosis was decreased markedly to a lower level (12.6%) in treatment group than in the control (25.9%). The results suggested that CSSO was able to protect the cells and prevent from oxidative cell damage caused by reactive oxygen species, and this effect may be closely associated with the ω-3/ω-6 constituent ratio in CSSO.

Key words: compatibility safflower seed oil, oxidative damage, SH-SY5Y, MTT

WU di, WU Gui-rong, Abulizi ABUDULA, SHENG Lei, Bahaerguli KAHAER. Preventive Effect of Different ω-3/ω-6 Constituent Ratio of Compatibility Safflower Seed Oil on SH-SY5Y Cells against Oxidative Cell Damage[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2011, 23(3): 420-424.

References 7

1	Wu GR(吴桂荣),Mao XM(毛新民),Wang Y(王岩). Effect of safflower oil emulsion on lipid lowing. Chin J New Drug,2003,12(2):111.
2	Zang YR(张艳荣),Zhang YY(张雅媛),Li Y(李玉). Anti-thrombosis action of Agaricus blazei Murrill ω-6 polyunsaturated fatty acid on rats and mice. J Jilin Univ (Med Ed),2006,32:774-776.
3	Xu ZH(徐章华),Shao YF(邵玉芬).Effects of α-linolenic acid on rats`behavior, retina and liver, brain fatty acid composition.China Public health, 2002, 18(3): 301-303.
4	Mandem akers W,Morais VA,De Strooper B.A cell biological perspective on mitochondrial dysfunction in Parkinson disease and other neurodegenerative disease. J Cell Sci, 2007,(10):1707-1716.
5	Chalon S,Vancassel S,Zimmer L,et al. Polyunsaturated fatty acids and cerebral function:focus on monoaminergic neurotransmission.Lipids,2001,36(9):937-944.
6	Rogers SL,Farlow MR,Doody RS,et al.A-4-week,double-blind,placebo-control ledtrial of donepezilin patients with Alzheimer’s disease.Neurology,1998,50(1):136.
7	Lim GP,Chu T,Yang F,et al.The curry spice curcumin reduces oxidative damage and amyloid pathology in an Alzheimer transgenic mouse.J Neurosci,2001,21:8370-8377.

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