Abstract:

The aim of this study was to investigate the analgesic, sedative and anti-inflammatory effects of the supercritical extract from Libanotis buchtormensis (SELB). Analgesic experiments were assessed by hot-plate test and turning test in mice. Sedative experiments were assessed by sleep-promotion test in combination with pentobarbital sodium and autonomic activities test in mice. Anti-inflammatory effects were assessed by the methods of albumn induced toes inflammation of rats and xylene induced ear edema of mice. The results showed that all dose groups of the SELB improved the pain threshold and inhibition ratio of stretching significantly in mice. In addition, the high and meddle dose groups of the SELB could significantly enhance hypnotic effect of pentobarbital sodium while all dose groups of the SELB inhibited the autonomic activities time notably in mice. And the high and meddle dose groups of the SELB inhibited toes tumefaction causing by albumen significantly in rats while all dose groups of the SELB could significantly inhibit the auricular edema causing by dimethylbenzene in mice. The SELB has analgesic, sedative and anti-inflammatory effects, which is coincident with the viewpoint of the Chinese Traditional Medicine and the clinical application of L. buchtormensis.

Key words: Libanotis buchtormensis (Fisch) DC., supercritical extract, analgesic, sedative, anti-inflammatory