Contents Analysis of Gentiopicroside in Wild and Tissue Culture Seedlings of Gentiana rigescens

NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2011, Vol. 23 ›› Issue (3): 482-485.

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Contents Analysis of Gentiopicroside in Wild and Tissue Culture Seedlings of Gentiana rigescens

ZHU Hong-tao1,2, ZHENG Chuan-wei2, ZHAO Ping2,3, LI Yuan1,4, YANG Chong-ren2, ZHANG Ying-jun2*

1College of Plant Protection, Yunnan Agricultural University, Kunming 650201,China; 2State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China; 3Faculty of Resources, Southwest Forestry University, Kunming 650224, China; 4College of Resources and Environment, Yunnan Agricultural University, Kunming 650201,China

Received:2009-09-25 Online:2011-06-25 Published:2011-08-08

Abstract

Abstract:

Tissue culture seedlings of Gentiana rigescens were carried out in the MS’s media with NAA, 6-BA and KT. The contents of gentiopicroside in leaves, stems and roots of tissue culture seedlings and wild plant were analyzed by HPLC. It was noticed that plentiful gentiopicroside accumulates in roots of wild plant, but hardly in roots of tissue culture seedlings. However, the content of gentiopicroside in leaves of tissue culture seedlings was higher or almost same as that in the wild roots. The results suggested that gentiopicroside was synthesized in the leaves and stored in the leaves simultaneously.

Key words: Gentiana rigescens, gentiopicroside, HPLC analysis

CLC Number:

Q946

ZHU Hong-tao, ZHENG Chuan-wei, ZHAO Ping, LI Yuan, YANG Chong-ren, ZHANG Ying-ju. Contents Analysis of Gentiopicroside in Wild and Tissue Culture Seedlings of Gentiana rigescens[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2011, 23(3): 482-485.

References 14

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Contents Analysis of Gentiopicroside in Wild and Tissue Culture Seedlings of Gentiana rigescens

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